PNP antibody (AA 142-153)

Catalog No. ABIN1590092

The Goat Polyclonal anti-PNP antibody has been validated for WB, ELISA and IHC. It is suitable to detect PNP in samples from Human.

Quick Overview for PNP antibody (AA 142-153) (ABIN1590092)

Target: PNP (Purine Nucleoside Phosphorylase (PNP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PNP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PNP Antibody

Binding Specificity: AA 142-153

Purpose: PNP (aa142-153)

Sequence: SGQNPLRGPN DE

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil and Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 30 kDa band observed in lysates of Human Liver, Erythrocytes and of cell lines Daudi, Jurkat and K562 (calculated MW of 32.1 kDa according to NP_000261.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PNP

Target: PNP (Purine Nucleoside Phosphorylase (PNP))

Alternative Name: PNP

Background: PNP, purine nucleoside phosphorylase, NP, PRO1837, PUNP, inosine phosphorylase, purine-nucleoside:orthophosphate ribosyltransferase

Gene ID: 4860

NCBI Accession: NP_000261

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Ribonucleoside Biosynthetic Process, Positive Regulation of Response to DNA Damage Stimulus